Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Wyatt
Date created: 2007-10-19 11:00:00
Date modified: 2015-08-31 04:08:04
AIP sensor infrastructure (agrC + agrA from S. aureus with RBSes)
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_I746100_sequence (Version 1) |
Description
This part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism.In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP.
This part contains the coding regions for group I agrC and agrA (in that order) from S. aureus strain NCTC8325, prefixed by the B0034 RBS and separated by a BamHI site.
Notes
N/ASource
The original sequences for agrC and agrA were taken from the S. aureus Sequencing Project (results for strain NCTC8325) at Oklahoma University (http://www.genome.ou.edu/staph.html). The sequences were then codon-optimised for E. coli using GeneDesigner from DNA2.0 (http://www.dna20.com), and then synthesised by that company.| Sequence Annotation | Location | Component / Role(s) |
| B0034-sequence RBS B0034-sequence RBS BamHI site agrC coding sequence agrA coding sequence | 1,12 1273,1284 1267,1272 19,1266 1291,1920 | feature/rbs ribosome_entry_site feature/rbs ribosome_entry_site feature/misc sequence_feature CDS feature/cds CDS feature/cds |