Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stefan Milde
Date created: 2008-09-29 11:00:00
Date modified: 2015-08-31 04:08:05
P7 GFP
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_I746917_sequence (Version 1) |
Description
Coding region of P7 GFP (Fisher et al(2008): "Laboratory evolution of fast-folding green fluorescent protein using secretory pathway quality control", PLoS ONE 3(6))P7 GFP carries the following amino acid changes as compared to mut3, the "standard" GFP used in the majority of registry constructs:
F64L, G65A, V68L, N105Y, E124V, Y145F
Its in vivo properties are the same as those of mut3 GFP (for improved in vivo properties see superfolder GFP: I746916). However, it is more stable in vitro and resists denaturation better than mut3. Additionally it refolds (after denaturation) at a much faster rate than does mut3 GFP - hence it might be useful for in vitro applications.
It has been used in the following constructs:
Driven by pBAD and T7 promoters: I746904 and I746906 respectively.
A 6-his tagged version for purification exists and is driven by pBAD or T7 as well: I746905 and I746907 are the respective part numbers.
Notes
PCR was carried out from plasmid DNA followed by standard assembly into several constructs.Source
P7 GFP was created and described by: Fisher et al(2008): "Laboratory evolution of fast-folding green fluorescent protein using secretory pathway quality control", PLoS ONE 3(6)It was biobricked by PCR and standard assembly from DNA kindly provided by Dr Adam C Fisher, Cornell University, New York.