BBa_I746918

BBa_I746918 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I746918
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stefan Milde
Date created: 2008-09-29 11:00:00
Date modified: 2015-08-31 04:08:05

mut3b GFP with 6-his tag



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_I746918_sequence (Version 1)

    Description

    Coding region of mut3b with C-terminal 6-his tag for assembly into constructs that allow 6-his purification of mut3b GFP.

    Notes

    2 stop codons were removed from the mut3b coding sequence and reinserted after the 6-his tag.

    Source

    The part was obtained by PCR from mut3b GFP from the 2007 parts distribution.

    Sequence Annotation Location Component / Role(s)
    start
    mut3b coding region
    6-his tag
    stop
    		1,3
    1,714
    715,732
    733,738
    		feature/start start_codon
    CDS feature/protein
    feature/tag tag
    stop_codon feature/stop

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_I746918/1
     

    Attachments

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