BBa_I746919

BBa_I746919 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I746919
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stefan Milde
Date created: 2008-09-29 11:00:00
Date modified: 2015-08-31 04:08:05

superfolder GFP with 6-his tag



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_I746919_sequence (Version 1)

Description

Coding region of superfolder GFP (see part description of I746916 for source and other information about superfolder GFP) with C-terminal 6-his tag for assembly into constructs that allow 6-his purification of this GFP variant for in vitro analysis.

Notes

2 stop codons were removed from the superfolder GFP coding region and reinserted after the 6-his tag.

Source

The part was obtained by PCR (with primers including the 6-his tag ) of the superfolder GFP coding region from part I746908.

Sequence Annotation Location Component / Role(s)
start
stop
6-his tag
superfolder GFP coding region
1,3
733,738
715,732
1,714
start_codon feature/start
feature/stop stop_codon
feature/tag tag
CDS feature/protein
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I746919/1