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BBa_I746920

BBa_I746920 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I746920

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Stefan Milde

Date created: 2008-09-29 11:00:00

Date modified: 2015-08-31 04:08:05

P7 GFP with 6-his tag

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Details

Types	DnaRegion
Roles	CDS Coding
Sequences	BBa_I746920_sequence (Version 1)

Description

Coding region of P7 GFP (see part description of I746917 for source and other information about P7 GFP) with C-terminal 6-his tag for assembly into constructs that allow 6-his purification of this GFP variant for in vitro analysis.

Notes

2 stop codons were removed from the P7 GFP coding region and reinserted after the 6-his tag.

Source

The part was obtained by PCR (with primers including the 6-his tag ) of the P7 GFP coding region from part I746904.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start stop P7 GFP coding region 6-his tag	1,3 733,738 1,714 715,732	feature/start start_codon stop_codon feature/stop CDS feature/protein tag feature/tag