Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Phillip Dodson
Date created: 2007-10-20 11:00:00
Date modified: 2015-08-31 04:08:05
Gas Vesicle polycistonic gene
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_I750016_sequence (Version 1) |
Description
This 5.7Kbp part contains 11 open reading frames coding for gas vesicle genes (gvpB,R,N,F,G,L,S,K,J,T and U) from Bacillus megaterium VT1660 AF053765.Promotion of the sequence results in expression of gas vesicles which slow the rate of settling of the cells in Saline.
Notes
Site directed mutagenesis was performed in four rounds to remove 3 PstI sites and one EcoRI site from gvpL in the sequence.Primers with biobrick prefix and suffix were then used in a PCR reaction to amplify the part.
Finally BBa_J61035 was used to provide the plasmid backbone which introduced a RBS at the front of the part.
Source
This 5.7Kbp part contains 11 open reading frames coding for gas vesicle genes (gvpB,R,N,F,G,L,S,K,J,T and U) from Bacillus megaterium VT1660 AF053765.Original AF053765 DNA in pBluescriptIIKS plasmid (pNL29 in "Gas Vesicle genes identified in Bacillus megaterium and Functional Expression in Escherichia coli, Ning Li and Maura C. Cannon Jounal of bacteriology May 1998 p2450-2458).
| Sequence Annotation | Location | Component / Role(s) |
| b0034 gvpB gvpR gvpN gvpF gvpG gvpL gvpS gvpK gvpJ gvpT gvpU gvpL g213a gvpL g318a gvpL g351a gvpL g696a 10 repeats of 40bp induced durring mutagenesis c->a t->c t->c t->a t->a g->a g->a First copy of 40bp section | 1,12 19,285 361,627 656,1583 1597,2364 2364,2632 2633,3842 3860,4147 4101,4385 4407,4709 4746,5624 5648,6064 2845,2845 2950,2950 2983,2983 3328,3328 3344,3743 718,718 2703,2703 2772,2772 3222,3222 4532,4532 5070,5070 5133,5133 3304,3343 | feature/rbs ribosome_entry_site feature/cds CDS CDS feature/cds CDS feature/cds CDS feature/cds CDS feature/cds feature/cds CDS feature/cds CDS feature/cds CDS feature/cds CDS CDS feature/cds CDS feature/cds point_mutation feature/s_mutation feature/s_mutation point_mutation feature/s_mutation point_mutation feature/s_mutation point_mutation feature/misc sequence_feature point_mutation feature/s_mutation feature/s_mutation point_mutation feature/s_mutation point_mutation sequence_alteration feature/mutation point_mutation feature/s_mutation feature/s_mutation point_mutation point_mutation feature/s_mutation feature/misc sequence_feature |