BBa_I751502

BBa_I751502 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I751502
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kenichiro Iwasaki
Date created: 2007-10-24 11:00:00
Date modified: 2015-08-31 04:08:06

plux-lac hybrid promoter



    • Details

    Types
    DnaRegion

    Roles
    Regulatory

    promoter

    Sequences BBa_I751502_sequence (Version 1)

    Description

    sense TWO INPUTS, activation by AHL and repression by LacI.

    Notes

    Compared with existing BioBrick hybrid promoter R0065, this promoter is different in that its sequence is much shorter with lux box and -35 region overlapping, and that it is repressed by LacI, not cI.
    Also, the output is clearly detected as shown in Fig. 3.

    Sequence Annotation Location Component / Role(s)
    plac
    -35
    -10
    O1
    O1
    luxR binding site
    		20,74
    20,26
    42,48
    27,41
    49,74
    1,19
    		feature/promoter promoter
    sequence_feature feature/misc
    sequence_feature feature/misc
    feature/binding non_covalent_binding_site
    feature/binding non_covalent_binding_site
    feature/binding non_covalent_binding_site

    igem#experience
    Works
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_I751502/1
     

    Attachments

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