Types | DnaRegion
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Roles | Reporter
engineered_region
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Description
Reporter construct for signaling circuit. GFP under the control of the lexAopMLP promoter and a SV40 polyA tail.
Notes
LexAop is a repeated sequence which results in multiple products. Isolate the largest fragment for gel isolation
Source
LexAopMLP (minimal promoter) from pSH1834 plasmid
GFP from PWPI lentiviral plasmid
SV40PA from pcDNA3.1 zeo (+) plasmid