Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Omar Khan
Date created: 2007-10-24 11:00:00
Date modified: 2015-08-31 04:08:07
mLexA-dnaEn-p85alpha
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_I756031_sequence (Version 1) |
Description
p85alpha is a subunit of PI-3K. It is linked, at its N terminus with mLexA-dnaEn. This construct was cloned into pcDNA3.1(+)Zeo with the sites: XbaI and EcoRV.Notes
A flexible GS Linker (GGGGS)x5 (or more) is required between the dnaEc and Syk. This was quite difficult to clone in with the FseI site used in the part.Source
p85 from OpenBiosystems (Clone ID: 3979333)mLexA from pEG202
dnaEn from dnaE gene of Synechocystis sp.(split inteins)