BBa_I756033

BBa_I756033 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I756033

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jennifer Lei

Date created: 2007-10-24 11:00:00

Date modified: 2015-08-31 04:08:07

CD11a

Details

• Types: DnaRegion
• Roles: engineered_region; Signalling
• Sequences: BBa_I756033_sequence (Version 1)

Description

LFA1 is a human transmembrane receptor composed of CD18 and CD11a which bind to ICAM. In acute myocardial infarction ICAM is exposed on the cell surface and therefore is one of the ideal targets for repairing damaged heart tissue.

To transmit the signal, we used a split ubiquitin system (Cub and NubG) that will reconstitute mLexA-VP16 in vivo. MLexA-VP16 translocates to nucleus where is functions as the switch to upregulate expression of the rescue circuit, death circuit and cell adhesion circuit via the binding to the lexA operator minimal promoter.

Notes

none

Source

kpn1 (1-6bp)

kozak (7-12bp)

CD11a from pCDL1 (13-3525bp) Larson, RS et al. J Cell Bio, 108(2): 703-12, 1989

stop stop (3526-3531bp)

xba1 (3532-3537bp)

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I756033/1

Member of these Collections

• iGEM Parts Registry