Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: 2007 NYMU_Taiwan iGEM Team
Date created: 2007-10-18 11:00:00
Date modified: 2015-08-31 04:08:08
TAT signal+INS_B
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_I761003_sequence (Version 1) |
Description
Twin arginin signal peptide is an efficient protein export signal sequence of E. coli. The purpose of this construct is to allow E. coli export insulin automatically, obviate the need to extract insulin by biochemical method.Notes
To combine TAT signal and INS_B, we used two separate PCR reaction to amplify each DNA fragment. We design the reverse primer of TAT signal and the forward primer of INS_B to be complement to each other, so when we mixed the product of each PCR reaction together, than used the forward primer of TAT signal and the reverse primer of INS_B to conduct PCR reaction, we can isolate the combine DNA product of TAT signal and INS_B. (Method devised by Dr. Chang)Source
NCBI accession number: BC005255K12 E. coli genomic DNA
| Sequence Annotation | Location | Component / Role(s) |
| TAT peptide export signal Human insulin B chain | 4,88 89,180 | feature/conserved conserved_region CDS feature/cds |