BBa_I766605

BBa_I766605 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_I766605

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Eric Chou

Date created: 2007-08-15 11:00:00

Date modified: 2015-08-31 04:08:10

YopH-EE under medium constitutive promotor

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_I766605_sequence (Version 1)

Description

This device will express YopH, a pathogen derived reversible inhibitor (phosphotase) of Map Kinase Fus3 under the pCyc promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing a matching RR zipper. The effector is fused mCherry (RFP) on the N terminal.

Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonuclease AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.

Source

Composite Part

Components

Access	Instance	Definition
public	BBa_I766007	BBa_I766007
public	BBa_I766555	BBa_I766555
public	BBa_I766101	BBa_I766101
public	BBa_I766102	BBa_I766102

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I766007	1,1413	BBa_I766007
BBa_I766555	1422,1665	BBa_I766555
BBa_I766101	1672,2391	BBa_I766101
BBa_I766102	2400,2561	BBa_I766102

Other Properties

• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I766605/1

Member of these Collections

• iGEM Parts Registry