BBa_I766655

BBa_I766655 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I766655

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: alexander ng

Date created: 2007-08-15 11:00:00

Date modified: 2015-08-31 04:08:10

YopJ-EE under medium constitutive promotor

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_I766655_sequence (Version 1)

Description

This device will express YopJ, a pathogen derived irreversible inhibitor (acetylase) of MapKK Ste7 under the pCyc promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing a matching RR zipper. The effector is fused mCherry (RFP) on the N terminal.

Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonucleases AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.

Source

composite part

Components

Access	Instance	Definition
public	BBa_I766555	BBa_I766555
public	BBa_I766101	BBa_I766101
public	BBa_I766002	BBa_I766002
public	BBa_I766102	BBa_I766102
public	BBa_I766600	BBa_I766600

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I766555	1,244	BBa_I766555
BBa_I766101	251,970	BBa_I766101
BBa_I766002	979,1851	BBa_I766002
BBa_I766102	1860,2021	BBa_I766102
BBa_I766600	2030,2247	BBa_I766600

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I766655/1

Member of these Collections

• iGEM Parts Registry