BBa_I766665

BBa_I766665 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I766665

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: alexander ng

Date created: 2007-08-15 11:00:00

Date modified: 2015-08-31 04:08:10

YopJ-EE under strong constitutive promotor

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_I766665_sequence (Version 1)

Description

This device will express YopJ, a pathogen derived irreversible inhibitor (acetylase) of MapKK Ste7 under the pAdh promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing a matching RR zipper. The effector is fused mCherry (RFP) on the N terminal.

Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonucleases AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.

Source

composite part

Components

Access	Instance	Definition
public	BBa_I766556	BBa_I766556
public	BBa_I766101	BBa_I766101
public	BBa_I766002	BBa_I766002
public	BBa_I766102	BBa_I766102
public	BBa_I766600	BBa_I766600

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I766556	1,1501	BBa_I766556
BBa_I766101	1508,2227	BBa_I766101
BBa_I766002	2236,3108	BBa_I766002
BBa_I766102	3117,3278	BBa_I766102
BBa_I766600	3287,3504	BBa_I766600

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I766665/1

Member of these Collections

• iGEM Parts Registry