BBa_I766669

BBa_I766669 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I766669

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jimmy Jie Min Huang

Date created: 2007-08-15 11:00:00

Date modified: 2015-08-31 04:08:10

YopJ-RR under weak constitutive promotor

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_I766669_sequence (Version 1)

Description

This device will express YopJ, a pathogen derived irreversible inhibitor (acetylase) of MapKK Ste7 under the pSte5 promotor. The effector protein is fused to an RR leucine zipper and will be recruited to elements possessing the matching EE zipper. The effector is fused mCherry (RFP) on the N-terminal.

Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonuclease AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.

Source

Composite part.

Components

Access	Instance	Definition
public	BBa_I766557	BBa_I766557
public	BBa_I766002	BBa_I766002
public	BBa_I766101	BBa_I766101
public	BBa_I766103	BBa_I766103
public	BBa_I766600	BBa_I766600

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I766557	1,601	BBa_I766557
BBa_I766002	610,1482	BBa_I766002
BBa_I766101	1489,2208	BBa_I766101
BBa_I766103	2217,2378	BBa_I766103
BBa_I766600	2387,2604	BBa_I766600

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I766669/1

Member of these Collections

• iGEM Parts Registry