BBa_I766670

BBa_I766670 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I766670

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Eric Chou

Date created: 2007-08-15 11:00:00

Date modified: 2015-08-31 04:08:10

YopH-EE under medium constitutive promotor

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_I766670_sequence (Version 1)

Description

This device will express YopH, a pathogen derived irreversible inhibitor (phosphotase) of MapK Fus3 under the pCyc promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing the matching EE zipper. The effector is fused mCherry (RFP) on the N-terminal.

Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonuclease AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.

Source

conposite part

Components

Access	Instance	Definition
public	BBa_I766555	BBa_I766555
public	BBa_I766007	BBa_I766007
public	BBa_I766101	BBa_I766101
public	BBa_I766102	BBa_I766102
public	BBa_I766600	BBa_I766600

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I766555	1,244	BBa_I766555
BBa_I766007	253,1665	BBa_I766007
BBa_I766101	1672,2391	BBa_I766101
BBa_I766102	2400,2561	BBa_I766102
BBa_I766600	2570,2787	BBa_I766600

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I766670/1

Member of these Collections

• iGEM Parts Registry