BBa_I766671

BBa_I766671 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_I766671

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Eric Chou

Date created: 2007-08-15 11:00:00

Date modified: 2015-08-31 04:08:10

YopH-EE under weak constitutive promotor

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_I766671_sequence (Version 1)

Description

This device will express YopH, a pathogen derived irreversible inhibitor (phosphotase) of MapK Fus3 under the pSte5 promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing the matching EE zipper. The effector is fused mCherry (RFP) on the N-terminal.

Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonuclease AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.

Source

conposite part

Components

Access	Instance	Definition
public	BBa_I766555	BBa_I766555
public	BBa_I766557	BBa_I766557
public	BBa_I766101	BBa_I766101
public	BBa_I766102	BBa_I766102
public	BBa_I766600	BBa_I766600

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I766555	1,244	BBa_I766555
BBa_I766557	253,853	BBa_I766557
BBa_I766101	860,1579	BBa_I766101
BBa_I766102	1588,1749	BBa_I766102
BBa_I766600	1758,1975	BBa_I766600

Other Properties

• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I766671/1

Member of these Collections

• iGEM Parts Registry