BBa_I766672

BBa_I766672 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_I766672

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Eric Chou

Date created: 2007-08-15 11:00:00

Date modified: 2015-08-31 04:08:10

YopH-EE under strong constitutive promotor

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_I766672_sequence (Version 1)

Description

This device will express YopH, a pathogen derived irreversible inhibitor (phosphotase) of MapK Fus3 under the pAdh promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing the matching EE zipper. The effector is fused mCherry (RFP) on the N-terminal.

Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonuclease AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.

Source

conposite part

Components

Access	Instance	Definition
public	BBa_I766555	BBa_I766555
public	BBa_I766556	BBa_I766556
public	BBa_I766101	BBa_I766101
public	BBa_I766102	BBa_I766102
public	BBa_I766600	BBa_I766600

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I766555	1,244	BBa_I766555
BBa_I766556	253,1753	BBa_I766556
BBa_I766101	1760,2479	BBa_I766101
BBa_I766102	2488,2649	BBa_I766102
BBa_I766600	2658,2875	BBa_I766600

Other Properties

• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I766672/1

Member of these Collections

• iGEM Parts Registry