BBa_I766673

BBa_I766673 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I766673

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Eric Chou

Date created: 2007-08-15 11:00:00

Date modified: 2015-08-31 04:08:10

YopH-EE under strong constitutive promotor

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_I766673_sequence (Version 1)

Description

This device will express YopH, a pathogen derived irreversible inhibitor (phosphotase) of MapK Fus3 under the pAdh promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing the matching EE zipper. The effector is fused mCherry (RFP) on the N-terminal.

Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonuclease AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.

Source

conposite part

Components

Access	Instance	Definition
public	BBa_I766556	BBa_I766556
public	BBa_I766007	BBa_I766007
public	BBa_I766101	BBa_I766101
public	BBa_I766102	BBa_I766102
public	BBa_I766600	BBa_I766600

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I766556	1,1501	BBa_I766556
BBa_I766007	1510,2922	BBa_I766007
BBa_I766101	2929,3648	BBa_I766101
BBa_I766102	3657,3818	BBa_I766102
BBa_I766600	3827,4044	BBa_I766600

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I766673/1

Member of these Collections

• iGEM Parts Registry