BBa_I766676

BBa_I766676 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I766676

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Eric Chou

Date created: 2007-08-15 11:00:00

Date modified: 2015-08-31 04:08:10

YopH-EE under weak constitutive promotor

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_I766676_sequence (Version 1)

Description

This device will express YopH, a pathogen derived irreversible inhibitor (phosphotase) of MapK Fus3 under the pSte5 promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing the matching EE zipper. The effector is fused mCherry (RFP) on the N-terminal.

Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonuclease AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.

Source

conposite part

Components

Access	Instance	Definition
public	BBa_I766557	BBa_I766557
public	BBa_I766007	BBa_I766007
public	BBa_I766101	BBa_I766101
public	BBa_I766102	BBa_I766102
public	BBa_I766600	BBa_I766600

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I766557	1,601	BBa_I766557
BBa_I766007	610,2022	BBa_I766007
BBa_I766101	2029,2748	BBa_I766101
BBa_I766102	2757,2918	BBa_I766102
BBa_I766600	2927,3144	BBa_I766600

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I766676/1

Member of these Collections

• iGEM Parts Registry