Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matt Gemberling
Date created: 2005-12-04 12:00:00
Date modified: 2015-08-31 04:08:13
"On" Antiswitch and target EYFP in the same plasmid
| Types | DnaRegion |
| Roles | mature_transcript_region RNA |
| Sequences | BBa_J04085_sequence (Version 1) |
Description
This is an "On" antiswitch placed into the same plasmid as the target EYFP. Both have the same promoter. This will help to ensure a close ratio of Antiswitch to EYFP mRNA. This part was designed based on Smolke and Bayer (2005).Source
Adaptation of antiswitch design "S8" by Bayer and Smolke; article available at http://www.nature.com/nbt/journal/v23/n3/full/nbt1069.html| Sequence Annotation | Location | Component / Role(s) |
| R0010 Hammerhead Ribozyme Antiswitch Hammerhead Ribozyme B0015 I6060 R0010 EYFP B0015 | 1,200 201,253 254,308 309,358 359,487 488,1612 488,687 688,1450 1451,1612 | feature/promoter promoter sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/stop stop_codon engineered_region feature/BioBrick feature/promoter promoter feature/cds CDS stop_codon feature/stop |