BBa_J04676

BBa_J04676 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J04676

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Oscar Hernandez and Matt Gemberling

Date created: 2005-09-14 11:00:00

Date modified: 2015-08-31 04:08:14

R0010 with Anti-Sense Theophillline Induced Antiswitch ("Off Switch")

Details

• Types: DnaRegion
• Roles: mature_transcript_region; RNA
• Sequences: BBa_J04676_sequence (Version 1)

Description

This Promoter and Antiswitch construct is designed so that it can be attached to any form of EYFP or YFP coding region. The purpose of this piece is to regulate the expression of YFP in the presence of theophylline. In this construct, the presence of theophylline causes the antiswitch, which is initially bound up on itself (allowing expression), to release and unfold allowing it to bind to the YFP. This, in turn, halts the expression of YFP.

Notes

Adaptation of antiswitch design "S8" by Bayer and Smolke; article available at http://www.nature.com/nbt/journal/v23/n3/full/nbt1069.html

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
R0010 Promoter Region	1,200	feature/promoter, promoter
Hammerhead Riobozyme #1	201,253	stem_loop, feature/stem_loop
Antiswitch	254,327	conserved_region, feature/conserved
Hammerhead Riobozyme #2	328,378	feature/stem_loop, stem_loop
Antisense region (binds with target)	254,267	non_covalent_binding_site, feature/binding

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J04676/1

Member of these Collections

• //classic/rna/uncategorized
• iGEM Parts Registry