|Sequences|| BBa_J06503_sequence (Version 1)|
First attempt to mutate failed. Suspect the problem is a low mutation rate due to results of positive control. May be a result of the mutagenesis kit being old.
Second attempt to mutate succeeded. However, the sequencing had a lot of bad sequence at the beginings, so that the ind- mutation was not sequenced by either of the primers used. The ind- mutation was verified by the presence of a HindIII restrition site.
This part was never completely sequenced. The cI857 QPI was made by Blue Heron, and this was part was abandoned.
Hendrix, Roger W. et al., ed. Lambda II. New York: Cold Spring Harbor Laboratory, 1983.
ind- mutation on p. 626 (at bp 37589 of Lambda)
857 mutation on p. 628 (at bp 37742 of Lambda)
Made by introducing two point mutations to Lambda cI (BBa_C0051): G(352)->A for ind- and G(199)->A for 857 temperature sensitivity.