mCherry

BBa_J06505 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J06505
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ytwang
Date created: 2005-07-17 11:00:00
Date modified: 2015-08-31 04:08:18

monomeric RFP optimized for bacteria



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Reporter

    Sequences BBa_J06505_sequence (Version 1)

    Description

    mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.

    Notes


    Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends with the LVA tag using PCR.



    Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.



    Source

    mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible and with an LVA tag added.

    Sequence Annotation Location Component / Role(s)
    mCherry
    C->T (removing PstI site)
    LVA
    		1,708
    352,352
    709,717
    		feature/cds CDS
    feature/mutation sequence_alteration
    feature/tag tag

    igem#experience
    None
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J06505/1
     

    Attachments

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