BBa_J100000

BBa_J100000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J100000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eric Sawyer
Date created: 2011-05-23 11:00:00
Date modified: 2015-08-31 04:08:21

Cre with 8bp restriction sites and 1-Clause 2-SAT Problem Inserted



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_J100000_sequence (Version 1)

Description

Cre recombines DNA at lox sites (J61046). After the start codon is an AsiSI site, followed by a SAT problem, followed by an AscI site. If the tRNA K199002 OR the tRNA K199001 is expressed in the cell, then Cre is read in-frame. If neither tRNA is expressed, then Cre is frameshifted.

Notes

N/A

Source

N/A

Sequence Annotation Location Component / Role(s)
Start
AsiSI site
1 Clause 2-SAT
AscI site
Remainder of Cre Recombinase
1,3
4,11
13,23
28,35
36,1069
start_codon feature/start
sequence_feature feature/misc
feature/misc sequence_feature
feature/misc sequence_feature
CDS feature/cds
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J100000/1