BBa_J100014

BBa_J100014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Eric Sawyer

Date created: 2011-06-20 11:00:00

Date modified: 2015-08-31 04:08:21

LuxI with 2 Clause 2-SAT Problem

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_J100014_sequence (Version 1)

Description

LuxI is frameshifted by the addition of a SAT problem between the engineered restriction sites AsiSI and AscI. The correct reading frame is restored only if suppressor tRNA CCACU (K199002) or suppressor tRNA CUAGU (K199001) AND suppressor tRNA CGGUC (K199028) or suppressor tRNA CCACU (K199002) are present in the cell.

Notes

N/A

Source

N/A

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start	1,3	start_codon, feature/start
Stop	647,652	feature/stop, stop_codon
AsiSI Site	4,11	sequence_feature, feature/misc
SAT Problem	13,37	sequence_feature, feature/misc
AscI Site	42,49	sequence_feature, feature/misc

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100014/1

Member of these Collections

• iGEM Parts Registry