BBa_J100095

BBa_J100095 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J100095
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pooja Potharaju
Date created: 2012-09-12 11:00:00
Date modified: 2015-08-31 04:08:22

malE1 Maltose induced promoter.



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_J100095_sequence (Version 1)

Description

malE1- found a paper for a maltose induced promoter CGR_2367. Testing whether or not this promoter works without the maltose induced part. Found the sequence in the paper and plan to make an oligo with less than 70 base pairs, trimming out the sequence induced by maltose. This oligo will be placed in the plasmid in the presence of RFP to test if this promoter will in fact still glow red despite the inducing sequence being trimmed out. We do not expect the promoter to work without the maltose induced sequence.

Notes

The maltose induced sequence was cut out; testing the viability of lactose production of this remaining sequence.

Source

Isolation, evaluation and use of two strong, carbon source-inducible promoters from corynebacterium glutamincum
N. Okibe, N. Suzuki, M. Inui, and H. Yakawa
Published in Society for Applied Microbiology, Letters in Applied Microbiology copy right 2009

Sequence Annotation Location Component / Role(s)
-35
-10
31,36
53,58
non_covalent_binding_site feature/binding
feature/binding non_covalent_binding_site
igem#experience
Issues
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J100095/1