BBa_J100102

BBa_J100102 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J100102
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Malcolm Campbell
Date created: 2012-11-26 12:00:00
Date modified: 2015-08-31 04:08:22

high production blue chromoprotein



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_J100102_sequence (Version 1)

Description

This construct uses a strong promoter (P2: J119029) and a strong CDog RBS (BD24: J119028) to produce blue chromoprotein (K592009). This should always produce blue pigment with no induction.

Notes

We used Golden Gate Assembly to connect S05027 to K592009_BD18).

Source

synthesized by GeneArt.

Sequence Annotation Location Component / Role(s)
P2 (strong)
BD24 (strong CDog)
start of reporter
amilCP, blue
stop, stop
1,46
47,131
132,134
132,800
795,800
promoter feature/promoter
feature/rbs ribosome_entry_site
feature/start start_codon
feature/cds CDS
stop_codon feature/stop
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J100102/1