BBa_J100105

BBa_J100105 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100105

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Erin McGuire

Date created: 2013-01-29 12:00:00

Date modified: 2015-08-31 04:08:22

Low production of eCDM8

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_J100105_sequence (Version 1)

Description

Low promotor and low RBS (c-Dog). E. coli optimized caffeine demethylase with the same amino acid sequence as yCDM8 from Michener and Smolke (2012).

Notes

S05008 was ligated using Bsa1, so there is no scar between the two parts and Pst1.

Source

eCDM8 was synthesized de novo.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J119030	1,36	feature/BioBrick, engineered_region
-35	1,6	feature/misc, sequence_feature
-10	24,36	feature/misc, sequence_feature
BBa_J119028	37,124	engineered_region, feature/BioBrick
leader RBS	55,63	ribosome_entry_site, feature/rbs
Start	69,71	start_codon, feature/start
GOI RBS	107,115	ribosome_entry_site, feature/rbs
Stop	120,122	feature/stop, stop_codon
Start	122,124	start_codon, feature/start
BBa_S05031	1,124	feature/BioBrick, engineered_region
J100100	125,3295	feature/BioBrick, engineered_region
Bba_suffix	3275,3295	engineered_region, feature/BioBrick
Double Stop	3269,3274	stop_codon, feature/stop

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100105/1

Member of these Collections

• iGEM Parts Registry