Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erin McGuire
Date created: 2013-02-19 12:00:00
Date modified: 2015-08-31 04:08:22
Riboswitch and GFP +Low production of eCDM8
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_J100106_sequence (Version 1) |
Description
Low promotor and low RBS (c-Dog). E. coli optimized caffeine demethylase with the same amino acid sequence as yCDM8 from Michener and Smolke (2012). Theophylline sensitive riboswitch mediates the production of GFP.Notes
J100079 was ligated using Spe1 and J100105 using Xba1, so there is no scar between the two parts and EcoR1.Source
eCDM8 was synthesized de novo.| Sequence Annotation | Location | Component / Role(s) |
| T5 promoter lac operator scar constant sequence KpnI site Riboswitch RBS Start superfolder GFP BBa_J100079 -35 BBa_J119030 BBa_S05031 -10 BBa_J119028 leader RBS Start GOI RBS Stop Start J100100 Double Stop Bba_suffix BBa_J100105 | 1,53 54,74 75,80 82,96 97,102 102,162 146,149 160,162 160,879 1,879 888,893 888,923 888,1011 911,923 924,1011 942,950 956,958 994,1002 1007,1009 1009,1011 1012,4182 4156,4161 4162,4182 888,4182 | promoter feature/promoter feature/operator operator sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc ribosome_entry_site feature/rbs feature/start start_codon CDS feature/protein feature/BioBrick engineered_region feature/misc sequence_feature feature/BioBrick engineered_region feature/BioBrick engineered_region sequence_feature feature/misc engineered_region feature/BioBrick ribosome_entry_site feature/rbs start_codon feature/start ribosome_entry_site feature/rbs stop_codon feature/stop start_codon feature/start feature/BioBrick engineered_region stop_codon feature/stop feature/BioBrick engineered_region engineered_region feature/BioBrick |