BBa_J100117

BBa_J100117 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J100117
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erich Baker
Date created: 2013-07-23 11:00:00
Date modified: 2015-08-31 04:08:23

pSB1A2 w/ "A" at -32 upstream from RNAII



Types
DnaRegion

Roles
Plasmid_Backbone

plasmid_vector

Sequences BBa_J100117_sequence (Version 1)

Description

This is a modified version of plasmid pSB1A2 (http://parts.igem.org/Part:pSB1A2). The position 32 bases upstream from the beginning of the RNAII transcript had been changed from a "C" to an "A". Mutations at this position have been shown to affect plasmid copy number in Castagnoli, 1985 (http://www.ncbi.nlm.nih.gov/pubmed/3895161).

Notes

Part was created by ligating pSB1A2 iPCR product to annealed oligos with an "N" at the -32 position.

Source

pSB1A2 used came from the registry.

Sequence Annotation Location Component / Role(s)
Suffix
Prefix
rep (pMB1) (rev)
APr (Rev)
"A"
1,21
2058,2079
183,797
957,1827
782,782
engineered_region feature/BioBrick
engineered_region feature/BioBrick
feature/cds CDS
feature/cds CDS
feature/mutation sequence_alteration
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J100117/1