BBa_J100117

BBa_J100117 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100117

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Erich Baker

Date created: 2013-07-23 11:00:00

Date modified: 2015-08-31 04:08:23

pSB1A2 w/ "A" at -32 upstream from RNAII

Details

• Types: DnaRegion
• Roles: Plasmid_Backbone; plasmid_vector
• Sequences: BBa_J100117_sequence (Version 1)

Description

This is a modified version of plasmid pSB1A2 (http://parts.igem.org/Part:pSB1A2). The position 32 bases upstream from the beginning of the RNAII transcript had been changed from a "C" to an "A". Mutations at this position have been shown to affect plasmid copy number in Castagnoli, 1985 (http://www.ncbi.nlm.nih.gov/pubmed/3895161).

Notes

Part was created by ligating pSB1A2 iPCR product to annealed oligos with an "N" at the -32 position.

Source

pSB1A2 used came from the registry.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Suffix	1,21	engineered_region, feature/BioBrick
Prefix	2058,2079	engineered_region, feature/BioBrick
rep (pMB1) (rev)	183,797	feature/cds, CDS
APr (Rev)	957,1827	feature/cds, CDS
"A"	782,782	feature/mutation, sequence_alteration

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100117/1

Member of these Collections

• iGEM Parts Registry
• igem_plasmid_vectors