BBa_J100161

BBa_J100161 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100161

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Telavive Taye

Date created: 2014-06-18 11:00:00

Date modified: 2015-08-31 04:08:23

weak promoter+theophylline riboswitch+thyA

Details

• Types: DnaRegion
• Roles: engineered_region; Measurement
• Sequences: BBa_J100161_sequence (Version 1)

Description

This has a weak promoter (P4) instead of a strong promoter (T5) found in part J100136. This version is designed to make less mRNA to see if the thyA fitness module will stay off in the absence of theophylline.

Notes

none

Source

Part J100136 but replaced the promoter with the standard promoter P4 (J119030).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
P4 (weak)	1,36	promoter, feature/promoter
theophylline riboswitch D	37,142	feature/rbs, ribosome_entry_site
thyA (J100132)	143,937	CDS, feature/cds
stop	935,937	stop_codon, feature/stop

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100161/1

Member of these Collections

• iGEM Parts Registry