Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Monica Prudencio
Date created: 2014-12-05 12:00:00
Date modified: 2016-11-05 04:36:06
actClone Red
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_J100204_sequence (Version 1) |
Description
We designed indClone Red to test the power of inducers. The two BsaI sites allow the reverse primer and transcriptional terminator to be removed and an inducer to be ligated in their place, causing translation of RFP. This can be used in synthetic biology laboratories or for undergraduate introductory biology lab modules.Notes
The stop codons of the RBS and start codons of the proteins overlap to increase effective translation. This is cloned into pSB1A8 with modified Bba prefix so that there is only an EcoRI site upstream and a normal Bba suffix.Source
The two main indicator proteins are GFP (BBa_I746916) and RFP (BBa_E1010).| Sequence Annotation | Location | Component / Role(s) |
| EcoRI superfolding GFP RBS BD24 BsaI site P5 Promoter Terminator L3S2P21 BsaI site 3' half, ompC promoter RBS BD21 RFP PstI site | 1,6 7,726 726,811 817,822 823,858 867,927 928,933 935,972 973,1058 1058,1738 1739,1744 | sequence_feature feature/misc feature/protein CDS feature/rbs ribosome_entry_site feature/misc sequence_feature promoter feature/promoter sequence_feature feature/misc feature/misc sequence_feature feature/promoter promoter ribosome_entry_site feature/rbs feature/protein CDS feature/misc sequence_feature |