Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julia Preziosi
Date created: 2015-06-01 11:00:00
Date modified: 2015-08-31 04:08:24
tClone Red RS3shift
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_J100208_sequence (Version 1) |
Description
This is a hybrid taken from part J119361, with everything between the BsaI restriction sites removed, and a riboswitch put in its place. The riboswitch was taken from the paper "De novo design of a synthetic riboswitch that regulates transcription termination" by Wachsmuth et al. 2013.Notes
There is a third BsaI site located on the plasmid, pUC-IDT-Amp, which could interfere with the cutting and ligasing in of the final product.Source
J119361, Wachsmuth et al. 2013.| Sequence Annotation | Location | Component / Role(s) |
| P5 promoter RS3shift Riboswitch BD18 C dog RBS RFP | 1,36 41,140 145,229 230,910 | feature/promoter promoter stem_loop feature/stem_loop feature/rbs ribosome_entry_site CDS feature/cds |