Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julia Preziosi
Date created: 2015-06-23 11:00:00
Date modified: 2015-08-31 04:08:24
tCloneTet with Riboswitch 10shift
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_J100216_sequence (Version 1) |
Description
This is a hybrid taken from part J119374, with everything between the BsaI restriction sites removed, and a riboswitch put in its place. The riboswitch was taken from the paper "De novo design of a synthetic riboswitch that regulates transcription termination" by Wachsmuth et al. 2013. This transcriptional riboswitch is expected to be able to regulate the reporter gene (in this case, Tetracycline Resistance) which follows it. By binding to the ligand, theophylline, the riboswitch will be "on," and the gene will be expressed. The absence of theophylline should turn the switch "off" and the gene won't be expressed.Notes
The plasmid that this part is located in is pUC-BR, a plasmid modified from pUC-IDT-Amp with an errant BsaI site in the Ampicillin resistance gene removed.Source
Part BBa_J119374, and Wachsmuth et al. 2013, available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575828/ .| Sequence Annotation | Location | Component / Role(s) |
| P5 promoter Riboswitch 10shift BD18 C dog RBS TetA | 1,36 41,127 132,216 217,1413 | promoter feature/promoter stem_loop feature/stem_loop feature/rbs ribosome_entry_site CDS feature/cds |