BBa_J100218

BBa_J100218 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J100218
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julia Preziosi
Date created: 2015-06-23 11:00:00
Date modified: 2015-08-31 04:08:24

tCloneTet with Riboswitch 3shift



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_J100218_sequence (Version 1)

Description

This is a hybrid taken from part J119374, with everything between the BsaI restriction sites removed, and a riboswitch put in its place. The riboswitch was taken from the paper "De novo design of a synthetic riboswitch that regulates transcription termination" by Wachsmuth et al. 2013. This transcriptional riboswitch is expected to be able to regulate the reporter gene (in this case, Tetracycline Resistance) which follows it. By binding to the ligand, theophylline, the riboswitch will be "on," and the gene will be expressed. The absence of theophylline should turn the switch "off" and the gene won't be expressed.

Notes

The plasmid that this part is located in is pUC-BR, a plasmid modified from pUC-IDT-Amp with an errant BsaI site in the Ampicillin resistance gene removed.

Source

Part BBa_J119374, and Wachsmuth et al. 2013, available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575828/ .

Sequence Annotation Location Component / Role(s)
P5 promoter
Riboswitch 3shift
BD18 C dog RBS
TetA
1,36
41,140
145,229
230,1426
promoter feature/promoter
feature/stem_loop stem_loop
feature/rbs ribosome_entry_site
CDS feature/cds
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J100218/1