Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julia Preziosi
Date created: 2015-06-23 11:00:00
Date modified: 2015-08-31 04:08:24
tCloneTet with Riboswitch 4
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_J100220_sequence (Version 1) |
Description
Using Golden Gate Assembly, the transcriptional riboswitch RS4 (from Wachsmuth, et al.) was ligated into the destination vector tClone Tet (J119374) in place of the insert (between BsaI sites). This riboswitch is controlled by theophylline, allowing transcription if theophylline is present. However, it also has shown increased transcription levels in the presence of caffeine. Based on the previous research of Wachsmuth, et al., we do not expect this to be a very effective riboswitch (small dynamic range). We will be using it as a negative control.Notes
The plasmid that this part is located in is pUC-BR, a plasmid modified from pUC-IDT-Amp with an errant BsaI site in the Ampicillin resistance gene removed.Source
Part BBa_J119374, and Wachsmuth et al. 2013, available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575828/ .| Sequence Annotation | Location | Component / Role(s) |
| P5 promoter Riboswitch 4 BD18 C dog RBS TetA | 1,36 41,117 122,206 207,1403 | promoter feature/promoter stem_loop feature/stem_loop ribosome_entry_site feature/rbs feature/cds CDS |