Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julia Preziosi
Date created: 2015-07-06 11:00:00
Date modified: 2015-08-31 04:08:24
Xanthine Aptamer from PDB with RS3, riboswitch in tCloneRed
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_J100221_sequence (Version 1) |
Description
This part comes from the combination of a xanthine aptamer, as determined by the Protein Data Bank, with part of "Riboswitch 3" from Wachsmuth (the sequence minus its aptamer) to make a new riboswitch. This riboswitch was inserted into tCloneRed, and functions to express the reporter gene, RFP, when in the presence of its ligand, xanthine.Notes
We had to add the aptamer to a riboswitch so that we could get the selective termination via the stem-loop behavior. We therefore did't know how the total riboswitch would fold or if it would be a successful riboswitch.Aptamer alone = ggacauAUaaucgcguggauauggcacgcaagUuucUAccgggcaccguaaauguccgACUaugucc
Source
tCloneRed = BBa_J119361Riboswitch 3 = J100207; Wachsmuth et al. 2013, available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575828/ .
| Sequence Annotation | Location | Component / Role(s) |
| P5 promoter Xanthine Riboswitch RFP BD 18 C dog RBS | 1,36 41,146 236,916 151,235 | promoter feature/promoter stem_loop feature/stem_loop feature/cds CDS feature/rbs ribosome_entry_site |