Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julia Preziosi
Date created: 2015-07-06 11:00:00
Date modified: 2015-08-31 04:08:24
Theophylline Aptamer from PDB with RS3, riboswitch in tCloneRed
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_J100224_sequence (Version 1) |
Description
This part comes from the combination of a theophylline aptamer, as determined by the Protein Data Bank, with part of "Riboswitch 3" from Wachsmuth (the sequence minus its aptamer) to make a new riboswitch. This riboswitch was inserted into tCloneRed, and functions to express the reporter gene, RFP, when in the presence of its ligand, theophylline.Notes
We had to add the aptamer to a riboswitch so that we could get the selective termination via the stem-loop behavior. We therefore did't know how the total riboswitch would fold or if it would be a successful riboswitch.Aptamer alone = ggcgaUACcagccgaaaggccCuUggcAgcguc
Source
tCloneRed = BBa_J119361Riboswitch 3 = J100207; Wachsmuth et al. 2013, available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575828/ .
| Sequence Annotation | Location | Component / Role(s) |
| P5 promoter BD 18 C dog RBS Theophylline Riboswitch RFP | 1,36 117,201 41,112 202,882 | feature/promoter promoter feature/rbs ribosome_entry_site stem_loop feature/stem_loop CDS feature/cds |