BBa_J100237

BBa_J100237 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100237

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shuk Hang Li

Date created: 2015-10-07 11:00:00

Date modified: 2016-07-12 12:29:35

Original Nitrate Biosensor (O)

Details

• Types: DnaRegion
• Roles: engineered_region; Measurement
• Sequences: BBa_J100237_sequence (Version 1)

Description

This construct is built by inserting narG promoter sequence into pClone Red (J119137) through GGA. Nitrate induces the promoter, so RFP is expressed when nitrate is present.

Notes

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Source

narG promoter was obtained from E. coli K-12 MG1655.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
GFP Reverse	1,720	CDS, feature/cds
RFP	1118,1799	feature/cds, CDS
narG promoter	755,1022	promoter, feature/promoter
BD18 bicistron RBS	1032,1117	feature/rbs, ribosome_entry_site

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_J100237/1

Member of these Collections

• iGEM Parts Registry