BBa_J100240

BBa_J100240 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100240

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Monica Prudencio

Date created: 2016-01-21 12:00:00

Date modified: 2016-01-21 01:10:14

repClone with P5 Promoter

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_J100240_sequence (Version 1)

Description

We created this part as an intermediate step in the development of repClone. It will be used to test the functionality of mutations in repClone. We used Golden Gate Assembly to insert the P5 promoter into repClone (J100205). This would allow us to determine whether potential SNP's in the RFP coding region of repClone prevent RFP expression, or if the cells will still turn red. It can also be used to test the TetR operon, if TetR is produced and binds indiscriminately to P5 as it would bind to the Ptet promoter.

Notes

N/A

Source

J100205 (repClone) and J119031 (P5)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
TetR (C0041)	1,639	CDS, feature/cds
P5+BD24 (J119031+J119028)	640,763	feature/misc, sequence_feature
GFP - codon optimized	764,1483	CDS, feature/cds
RBS B0034	1490,1501	feature/rbs, ribosome_entry_site
sticky end	1512,1515	sequence_feature, feature/misc
P5 Promoter (J119031)	1516,1551	feature/promoter, promoter
sticky end	1552,1555	feature/misc, sequence_feature
RBS BD18 (J119024)	1556,1643	feature/rbs, ribosome_entry_site
RFP (E1010)	1641,2321	CDS, feature/cds

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100240/1

Member of these Collections

• iGEM Parts Registry