Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Owen Koucky
Date created: 2016-06-16 11:00:00
Date modified: 2016-06-17 08:13:27
pJC173b (Carlson et al. 2014)
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_J100265_sequence (Version 1) |
Description
In their 2014 paper "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., designed part J100265 to be used as an accessory plasmid in phage-assisted continuous evolution (PACE).Notes
See "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014Source
"Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014| Sequence Annotation | Location | Component / Role(s) |
| RepA SC101 Origin of Replication T7 T7 Promoter SD8 RBS geneIII LuxAB bla (AmpR) AmpR bla promoter | 1,951 955,1700 1785,1804 1785,1804 1826,1840 1841,3115 3115,5238 5318,6178 5312,5980 6179,6373 | feature/cds CDS feature/misc sequence_feature primer_binding_site feature/primer_binding feature/promoter promoter ribosome_entry_site feature/rbs CDS feature/cds feature/cds CDS CDS feature/cds CDS feature/cds feature/promoter promoter |