Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Owen Koucky
Date created: 2016-06-16 11:00:00
Date modified: 2016-06-17 10:07:20
SP-T7Promiscuous_L1-192.2 (Carlson et al. 2014)
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_J100269_sequence (Version 1) |
Description
In their 2014 paper "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., designed part J100269 to be used as a selection plasmid in phage-assisted continuous evolution (PACE).Notes
"Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014Source
"Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014| Sequence Annotation | Location | Component / Role(s) |
| geneII geneX geneV geneVII geneIX geneVIII geneIII promoter KanR M13 SP T7 RNAP SP-Promiscuous geneIII fragment geneVI geneI geneIV M13 Origin F1 Origin of Replication geneII promoter | 1,1233 898,1233 1245,1508 1510,1611 1608,1706 1703,1924 1910,1939 1987,2802 2823,5474 5495,5677 5680,6018 6020,7066 7044,8324 8312,8767 8329,8635 8758,8786 | CDS feature/cds CDS feature/cds feature/cds CDS CDS feature/cds CDS feature/cds CDS feature/cds promoter feature/promoter CDS feature/cds CDS feature/cds CDS feature/cds CDS feature/cds CDS feature/cds feature/cds CDS sequence_feature feature/misc sequence_feature feature/misc feature/promoter promoter |