BBa_J100270

BBa_J100270 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J100270
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Owen Koucky
Date created: 2016-06-16 11:00:00
Date modified: 2016-06-17 10:21:19

SP-T7Specific_L2-48.3 (Carlson et al. 2014)



Types
DnaRegion

Roles
plasmid

Plasmid

Sequences BBa_J100270_sequence (Version 1)

Description

In their 2014 paper "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., designed part J100270 to be used as a selection plasmid in phage-assisted continuous evolution (PACE).

Notes

"Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014

Source

"Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014

Sequence Annotation Location Component / Role(s)
geneII
geneX
geneV
geneVII
geneIX
geneVIII
geneIII promoter
KanR M13 SP
T7 RNAP SP-Specific
geneIII fragment
geneVI
geneI
geneIV
M13 Origin of Replicaiton
F1 Origin of Replication
geneII promoter
1,1233
898,1233
1245,1508
1510,1611
1608,1706
1703,1924
1910,1939
1987,2802
2823,5474
5495,5677
5680,6018
6020,7066
7044,8324
8312,8767
8329,8635
8758,8786
feature/cds CDS
feature/cds CDS
CDS feature/cds
CDS feature/cds
CDS feature/cds
CDS feature/cds
promoter feature/promoter
CDS feature/cds
CDS feature/cds
CDS feature/cds
feature/cds CDS
feature/cds CDS
CDS feature/cds
feature/misc sequence_feature
feature/misc sequence_feature
feature/promoter promoter
igem#status Planning  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_J100270/1