Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anna Buser
Date created: 2016-06-23 11:00:00
Date modified: 2016-06-24 09:45:50
tCLoneTet+PDB riboswitch with P4
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_J100276_sequence (Version 1) |
Description
The tCloneTet+PDB plasmid was obtained from part J100255 and was modified using Golden Gate Assembly to have a P4 promoter rather than the P5 promoter. The P4 promoter is known to have the lowest efficiency in its family of promoters.Notes
Used PCR to "cut out" the P5 promoter since there were no restriction enzyme sites surrounding it.Source
J100255| Sequence Annotation | Location | Component / Role(s) |
| P4 Promoter PDB riboswitch BD18 C Dog RBS TetA | 1,36 41,112 117,201 202,1398 | promoter feature/promoter stem_loop feature/stem_loop feature/rbs ribosome_entry_site feature/cds CDS |