Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anna Buser
Date created: 2016-06-23 11:00:00
Date modified: 2016-06-24 10:33:18
tCLone Tet+PDB riboswitch with P14 and BD2
| Types | DnaRegion |
| Roles | engineered_region Measurement |
| Sequences | BBa_J100278_sequence (Version 1) |
Description
The BD2 C Dog Ribosome Binding Site has a lower binding efficiency than BD18 and BD10. PCR and Golden Gate Assembly was used to cut out the BD18 C Dog RBS of part J100274 and insert in the BD2 C Dog RBS.Notes
Since there was only a 6 base difference between BD18 C Dog and BD2 C Dog, the PCR was used to insert in and amplify the new BD2 C Dog RBS rather than during GGA. GGA was still necessary to make the dsDNA into a plasmid.Source
J100274| Sequence Annotation | Location | Component / Role(s) |
| P14 PDB riboswitch BD2 C Dog RBS TetA | 1,36 41,112 117,201 202,1398 | promoter feature/promoter stem_loop feature/stem_loop feature/rbs ribosome_entry_site CDS feature/cds |