View

BBa_J100280

BBa_J100280 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J100280

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Anna Buser

Date created: 2016-06-23 11:00:00

Date modified: 2016-06-24 10:48:30

tClone Tet+PDB riboswitch with P9 and BD2

Back

Details

Types	DnaRegion
Roles	Measurement engineered_region
Sequences	BBa_J100280_sequence (Version 1)

Description

The BD2 C Dog Ribosome Binding Site has a lower binding efficiency than BD18 and BD10. PCR and Golden Gate Assembly was used to cut out the BD18 C Dog RBS of part J100275 and insert in the BD2 C Dog RBS.

Notes

Since there was only a 6 base difference between BD18 C Dog and BD10 C Dog, the PCR was used to insert in and amplify the new BD10 C Dog RBS rather than during GGA. GGA was still necessary to make the dsDNA into a plasmid.

Source

J100275

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
P9 PDB BD2 C Dog TetA	1,35 40,111 116,201 200,1397	promoter feature/promoter feature/stem_loop stem_loop feature/rbs ribosome_entry_site CDS feature/cds