BBa_J100285

BBa_J100285 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J100285
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dylan Maghini
Date created: 2016-07-07 11:00:00
Date modified: 2016-07-11 09:07:42

SPT7specific with Riboswitch C



Types
DnaRegion

Roles
plasmid

Plasmid

Sequences BBa_J100285_sequence (Version 1)

Description

This is the SPT7specific plasmid with translational riboswitch C inserted. It will be used in the positive and negative phases of the Campbell lab's phage assisted continuous evolution experiment. In the experiment, we will apply pressure on the theophylline riboswitch to evolve into a xanthine specific riboswitch.

Notes

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Source

This part comes from SPT7spec (J100270). The riboswitch comes from the Gallivan et al. paper titled "A family of synthetic riboswitches adopts a kinetic trapping mechanism" (2014).

Sequence Annotation Location Component / Role(s)
riboswitch C
KanR M13 SP
T7 RNAP SP
2808,2866
1987,2802
2870,5521
sequence_feature feature/misc
sequence_feature feature/misc
feature/misc sequence_feature
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J100285/1