Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hartlee Johnston
Date created: 2016-07-10 11:00:00
Date modified: 2016-07-11 12:31:37
pJC173b with gIII neg
| Types | DnaRegion |
| Roles | plasmid Plasmid |
| Sequences | BBa_J100288_sequence (Version 1) |
Description
In their 2014 paper "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., designed part J100265 (pJC173b) to be used as an accessory plasmid in phage-assisted continuous evolution (PACE). This DNA part comes from a collection of 8 plasmids numbers J100264 - J100271. PACE employs M13 phage and E. coli in a chemostat. This construct is based on J100265 and contains a deletion of 210 bp, encoding 70 amino acids, from gene III. The mutant gene III encodes a protein III that acts as a dominant negative for M13 phage reproduction.Notes
Used BbsI as a restriction enzyme because of the internal BsaI site.Source
Part was designed using BbsI and GGA to remove a 210 bp section from J100265.| Sequence Annotation | Location | Component / Role(s) |
| RepA SC101 Origin of Replication T7 promoter T7 SD8 RBS Gene III neg LuxAB AmpR bla promoter | 1,951 955,1700 1784,1803 1784,1803 1825,1839 1840,2903 2904,5027 5110,5769 5968,6162 | feature/cds CDS sequence_feature feature/misc feature/promoter promoter feature/primer_binding primer_binding_site feature/rbs ribosome_entry_site feature/cds CDS feature/cds CDS feature/cds CDS feature/promoter promoter |