Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuk Hang Li
Date created: 2016-07-11 11:00:00
Date modified: 2016-07-12 12:26:57
Pnar7 Nitrate Biosensor
| Types | DnaRegion |
| Roles | engineered_region Measurement |
| Sequences | BBa_J100289_sequence (Version 1) |
Description
This construct is built by modifying the promoter sequence of Original Nitrate Biosensor (J100237), which inserts narG promoter sequence into pClone Red (J119137) through GGA. The promoter sequence is modified by disrupting both Fis binding sites where an inhibitory regulator binds; moreover,since there is an overlap between the activator and inhibitor binding site, we had to make sure the activator can still bind on the same site. The modified promoter is inserted in to pClone Red (J119137). The goal is that nitrate induces the promoter, then RFP is expressed when nitrate is present. However, this promoter does not induce RFP production when nitrate is present.Notes
/Source
narGHJI operon| Sequence Annotation | Location | Component / Role(s) |
| Pnar7 promoter GFP Reverse RFP | 755,1025 1,720 1123,1803 | feature/promoter promoter feature/cds CDS CDS feature/cds |